While we considered the possibility that this unusual splice variant of VEGF might not be able to produce an angiogenic peptide, it is of interest that another rare splice variant of (mouse) VEGF mRNA has also been described that contains only the first 3 exons of VEGF. This splice variant (VEGF 115) has been shown to encode a peptide that is able to bind to VEGF receptor and activate mitogenesis of endothelial cells. Therefore, the unusual VEGF splice variant (VEGF 110) we observed in our studies, although seemingly rare and low in abundance, probably has the potential to produce a functional VEGF peptide. proventil inhaler
The recent approval by the Food and Drug Administration of Sildenafil citrate as the first oral therapy for erectile dysfunction has become a landmark for the development of pharmacotherapy for erectile dysfunction. However, all current pharmacologic treatments of erectile dysfunction consist of ‘‘on-demand’’ medications that produce temporary erections. At this time there is no curative treatment for erectile dysfunction. The work in this project is based on our conceptualization of the induction of vasculogenesis in the erectile tissue as a potential cure for the reduction of vascular elements that is frequently encountered in vascu-logenic erectile dysfunction. In this descriptive study, VEGF-A mRNA expression was surveyed in the rodent and human penis. The present characterization of the various VEGF isoforms in erectile tissues is motivated by our interest in defining the angiogenic growth factors that regulate penile vasculogenesis and in potentially exploiting these findings for therapeutic applications to treat human impotence. One could conceivably consider the therapeutic application of VEGF peptides to stimulate further penile vascular development as has been contemplated for other vascular insufficiency conditions. Likewise, use of VEGF expression vectors provides a novel gene-therapeutic approach for the curative treatment of erectile dysfunction, an approach that is being contemplated for other gene products involved in the response. By identifying the predominant angiogenic peptides naturally expressed in the erectile tissues of the penis, we are one step closer to this goal.