Elevated Levels of Soluble Adhesion Molecules in Serum of Patients With Diffuse Panbronchiolitis: Statistical AnalysisData were expressed as mean±SD. For comparison between groups, one-way analysis of variance (ANOVA) or paired t test was used. The correlation between two parameters was examined by Spearman’s rank correlation test. A probability of <5% was considered significant. viagra super active plus

Results Serum Levels of Soluble Selectins
The serum levels of sL-selectin in patients with DPB and bronchiectasis were significantly higher than in volunteer subjects (Fig 1A [top]). The serum levels of sE-selectin in patients with DPB were significantly higher than those in patients with bronchiectasis and volunteers (Fig IB [center]). The mean serum level of sP-selectin in patients with DPB was significantly higher than in healthy volunteers and patients with bronchiectasis (Fig 1C [bottom]).
Serum Levels of Soluble ICAM-1 and VCAM-1
The serum levels of sICAM-1 and sVCAM-1 in DPB patients were similar to those in patients with bronchiectasis, but significantly higher than in healthy volunteers (Fig 2).
Correlation Between Soluble Adhesion Molecules and BALF Cells
As shown in Table 1, the total number of cells recovered from BALF of DPB patients was higher than that of the control subjects and patients with bronchiectasis. In addition, the relative number of neutrophils in this fluid was also significantly higher in DPB patients than in the control group and patients with bronchiectasis. There was a significant correlation between the serum concentration of sE-selectin and percentage of neutrophils in BALF in all patients (n=37, r=0.50, p<0.01).
Correlation Between Soluble Adhesion Molecules and Clinical Parameters
A significant inverse relationship was noted between serum levels of sE-selectin and FEV1 (r— —0.41, p<0.05), FEV1% (r=0.40, p<0.05), and VC% (Fig 3) in DPB patients. There was, however, no significant relationship between serum levels of other soluble molecules and the clinical parameters, eg, Pa02, VC%, or FEV1% in the same patients. In addition, no significant correlation was observed between serum concentrations of these adhesion molecules and the above clinical parameters in bronchiectasis.
Table 1—Characteristics of BALF Cells

Healthy Volunteers (n = ll) Bronchiectasis(n=10) DPB(n=27)
Total cells, X10°/mL 1.3±0.7 2.0±1.011 13.3 ±15.1
Macrophages, % 84.7±6.9 59.5±31.3 12.5±15.3f
Lymphocytes, % 11.8±5.8 17.6±17.4!l 8.2±6.4
Neutrophils, % 2.4±4.4 22.7±24.4 79.3±17.8t

Figure 1. Serum concentrations of (A [top]): soluble L-selectin; (B [center]): E-selectin; and (C [bottom]): P-selectin in healthy subjects (n=15) and patients with bronchiectasis (n = 13) and DPB (n=27). Each point represents an individual subject. Open squares and vertical lines: mean±SD of each group.

Figure 1. Serum concentrations of (A [top]): soluble L-selectin; (B [center]): E-selectin; and (C [bottom]): P-selectin in healthy subjects (n=15) and patients with bronchiectasis (n = 13) and DPB (n=27). Each point represents an individual subject. Open squares and vertical lines: mean±SD of each group.

Figure 2. Serum concentrations of (A [top]): soluble ICAM-1; and (B [bottom,]): VCAM-1 in healthy subjects and patients with bronchiectasis and DPB. Each point represents an individual subject. Open squares and vertical lines: mean±SD of each group.

Figure 2. Serum concentrations of (A [top]): soluble ICAM-1; and (B [bottom,]): VCAM-1 in healthy subjects and patients with bronchiectasis and DPB. Each point represents an individual subject. Open squares and vertical lines: mean±SD of each group.

Figure 3. Correlation between soluble E-selectin concentrations in serum and VC% in DPB patients (n=27).

Figure 3. Correlation between soluble E-selectin concentrations in serum and VC% in DPB patients (n=27).