This study did not evaluate urinary excretion or dietary intake of thiamine, but previous reports have suggested that thiamine loss during furosemide therapy may occur due to increased urinary excretion. Interestingly, a recent study found that furosemide and digoxin significantly decreased the thiamine uptake by cardiac cells in vitro. This effect was determined to be […]
In our study, patients with more severe CHF, as indicated by lower LVEF, tended to be more thiamine deficient, but this was not statistically significant. However, two studies found LVEF to be adversely affected by biochemical evidence of thiamine deficiency. The studies further went on to describe that upon thiamine replenishment, LVEF improved by 18% […]
In this study, biochemical evidence of severe thiamine deficiency was found in 96% (24 of 25) of patients receiving 80 mg or more of furosemide a day. Of these patients, 96% were receiving furosemide for more than three months. This finding supports previous reports of a high prevalence of thiamine deficiency in patients receiving at […]
A high TPPE percentage (greater than 25%), indicating a severely deficient thiamine status, was found in 24 of 25 (96%) in group A patients receiving 80 mg or more of furosemide (Figure 1) and in four of the seven (57%) low dose patients (Figure 2). The mean TPPE percentage in high dose patients (group A) […]
Study subjects In total, 32 subjects with chronic CHF were enrolled in the study after meeting the inclusion criteria. The study compromised 23 men and nine women; the mean age was 65±14 years. Subjects were placed in either group A or group B based on their furosemide dose. The average dose of furosemide in group […]
Hemoglobin was measured after dilution using a spectropho-tometrical cooximeter. Working reagents, sufficient for one complete day, were prepared fresh for the Cobas Mira analyzer. The reagents included in this assay were ribose-5-phosphate, xylose-5-phosphate, NADH, thiamine pyrophosphate (TPP) and glycerol 3-phosphate dehydrogenase/triose phosphate isomerase combined suspension.
Biochemical measurement Thiamine status was evaluated by a modified nicotinamide-adenosine dinucleotide (NADH) erythrocyte TK assay . The reactants used were similar to those used in the method originally used by Bayoumi and Rosalki which was adapted for use on the Cobas Fara (Roche, USA) by Wenzhe et al . The assay methodology and specificity were […]