Spermatozoa Category

The presence of plasma membrane NADH-oxidases capable of generating ROS has been described in a wide vamnsrrrepit lqdpeakypl pliekekish ntrrfrfglp spdhvlglpv gnyvqllaki dnelvvrayt pvssdddrgf vdliikiyfk nvhpqypeg gkmtqylen mkigetiffr gprgrlfyhgp mlgirpdq tsepkktlad hlgmiaggtg itpmlqlirh itkdpsdrtrm slifanqtee dilvrkeleei arthpdqfdl wytldrppig wkyssgfvta dmikehlpp pakstlilvc riety of cell types, including fibroblasts, glomerular mesangial cells, B-lymphocytes, leukocytes, endothelial cells, and […]

Generation of an NADH-Dependent Lucigenin Chemiluminescant Signal in Human Spermatozoa Human sperm preparations demonstrated an NADH-dependent lucigenin chemiluminescence signal that was cell number dependent (Fig. 1A; P < 0.001). Addition of superoxide dismutase (SOD1; Fig. 1B) to the system inhibited the redox activity (P < 0.01), suggesting the involvement of O2“. At this dose of […]

Chemicals All chemicals were purchased from Sigma-Aldrich (Castle Hill, NSW Australia) with the exception of catalase (Calbiochem, Melbourne, Australia; specific activity 30 000 U/mg), wSt-1 (AusPep, Parkville, VIC, Australia), MCLA (Molecular Probes, Castle Hill, NSW, Australia), and anti-GFP (green fluorescent protein) antibodies (GE Healthcare, Castle Hill, NSW, Australia). Preparation of Human Spermatozoa The study population […]

Reactive oxygen species (ROS) are now accepted as playing pivotal roles in many forms of cell signaling. For example, overexpression of NADPH-oxidase 1 within NIH 3T3 cells leads to an increase in proliferation and invasiveness via mechanisms that can be reversed by the concomitant overexpression of catalase. Reactive oxygen species are also involved in mediating […]