Spermatogonia Category

The involvement of the pituitary gland in spermatogo-nial proliferation has been addressed in numerous studies. As most of these studies focused on stem cell differentiation, the effects on stem cell self-renewing division were not determined due to the difficulty in distinguishing the type of division by morphological assay. To address this issue, we used two […]

Pituitary Gland Enhances Stem Cell Proliferation During Postnatal Testicular Development In the first set of experiments, we examined the effect of the pituitary gland on stem cells in the immature postnatal testis. For this purpose, neonatal MT-lacZ mice were injected with leuprorelin, which reduces the levels of LH and FSH. The injected mice were returned […]

Animals In the first set of experiments, donor cells were collected from the testes of transgenic mice of the lineage C57BL/6J-TgN(MTnlacZ)204Bri (designated as MT-lacZ), which were purchased from Jackson Laboratory (Bar Harbor, ME). The MT-lacZ transgene is thought to be expressed initially in primary spermatocytes. The donor mice were injected with leuprorelin acetate (0.19 mg […]

Spermatogenesis depends on a small population of cells that are called spermatogonial stem cells. There are 20 00030 000 stem cells in the mouse testis, and these cells produce sperm throughout the life span of the animal. An important characteristic of stem cells is their ability to self-renew. It is generally believed that stem cells […]