Spermatocytes Category

In related studies using CAT-reporter gene transcription driven by the human histone H1t promoter, it was shown that the H1/CCAAT box and its cognate binding protein are essential for the elevated gene transcription seen during S-phase of the cell cycle in transiently transfected HeLa cells. However, our recent experiments have shown that transcription driven by […]

The TE element was deleted from the proximal promoter of the rat H1t gene (Fig. 2A) and was replaced with a stuffer fragment of DNA to conserve spacing between the other promoter elements. This stuffer fragment had previously been shown not to compete with the TE element for protein binding. The substitution abolished transcription of […]

Transgenic mouse technology has been used to characterize many tissue-specific promoters. It allows researchers to study regulation of gene transcription in vivo, confirming the importance of conserved regulatory sequences by mutation analysis and revealing novel regulatory sequences by means of serial deletion of flanking regions. buy ortho tri-cyclen H1t is one of seven known linker […]

To determine whether the rat H1t gene was located in a transcriptionally favorable chromatin environment, and to verify loading accuracy, the same RNA samples were subjected to further S1 analysis to detect transcription of the neighboring rat H4t gene. Several mice had relatively high steady-state levels of mRNA that hybridized to the rat H4t probe […]

Since the rat but not the mouse gene contains a Pst I restriction site between these primers, the amplified products were digested with Pst I to confirm the presence of the rat genomic DNA sequence. In Figure 4, lanes marked with a plus, showing digested samples, are compared to lanes marked with a minus, showing […]

Wild-Type but Not the Mutant Rat Hit Gene Was Transcribed in Mice Bearing the Transgene There were at least two possible outcomes in this transgenic mouse study. If the TE element participates in silencing transcription of the H1t gene in nongerminal cells in vivo, we expected to see possibly inappropriate basal or enhanced transcription in […]

The mutant element was most likely shortened when S1 nuclease was used to form blunt ends from the promoter that had been cut with the restriction enzymes £coNI and Avr II. A plasmid designated pHD1m (Fig. 2B) containing the H1t gene with this replacement was constructed first. The Sca I fragment containing this mutant sequence […]