Sperm Category

Another model proposes that steroid receptors, bound to their cognate DNA element, lead to a disruption of phased nucleosomes by interacting with accessory factors such as CREB binding protein (CBP), steroid receptor coactivator 1 (SRC-1), p300/CBP associated factor (PCAF), and androgen receptor associated protein (ARA70). In a first step, the recruitment of coactivators that have […]

One the most interesting features of the mE-RABP ARR -543/+26 is that it is activated by AR and not by GR in HeLa and PC-3 cells. Hormonal specificity may depend, in part, on the affinity of the steroid receptor for its cognate response element. The AR binding site 2 of the probasin ARR binds differentially […]

The replacement of the proximal mE-RABP promoter with the heterologous tk promoter (construct —543/—88) nearly eliminated the androgen-induced response even in the presence of both ARBS-1 and ARBS-0. Within the in-tron 1 of the C3(1) PSBP gene, the functional activity of a GRE/ARE-like sequence (named core II or site C) is enhanced by surrounding sequences. […]

Within the probasin gene promoter, two AR binding sites, named ARBS-1 and ARBS-2, cooperate to give rise to an efficient androgen responsiveness. Synergism between multiple AR binding sites was also described within the first intron of the cysteine-rich protein 2 (CRP2) gene, the distal promoter of the secretory component (sc) gene, and the distal and […]

In this study, the characterization of an androgen-specific response region located within the first 600 bp of the mE-RABP gene promoter is described. We have recently shown that a 5 kb DNA fragment of the 5′ flanking region of the mE-RABP gene, linked to the CAT reporter gene, mimics the tissue-specific, cell-specific, and androgen-regulated expression […]

Altogether, these results indicate that ARBS-1 is likely the most important motif in the androgen response region (-543/+26) of the mE-RABP gene and that although ARBS-0 is not essential for the androgen-specific response, it is required to further increase the magnitude of response to androgen treatment. ARBS-1 Can Act as a Weak Androgen Response Element […]

To confirm the specificity of AR/DNA interaction, EMSA was carried out using double-stranded oligonucleotides encompassing the protected area as probes (Fig. 3, C through E). When labeled duplex oligomers encompassing ARBS-1 were used as probes, addition of the GST-AR2 fusion protein to the incubation mixture led to the formation of retarded complexes (Fig. 3C). The […]