Ribonucleic Acid Category

While we considered the possibility that this unusual splice variant of VEGF might not be able to produce an angiogenic peptide, it is of interest that another rare splice variant of (mouse) VEGF mRNA has also been described that contains only the first 3 exons of VEGF. This splice variant (VEGF 115) has been shown […]

Whereas we have confirmed that the rat lung expresses most abundantly an mRNA encoding the 188 splice variant, the rat penis, in contrast, expresses the 164 splice variant of VeGf-A mRNA at greatest abundance. Other VEGF splice variants, including the previously described 120 splice variant, are also expressed at much lower levels by the lung […]

Erectile function is dependent on the development of an appropriate penile vasculature and maintenance of an effective blood supply to the erectile tissues of the penis. The development and growth of a vascular system are referred to as angiogenesis and vasculogenesis. These processes are governed by several types of growth factors, including basic FGF (bFGF) […]

The probes were individually hybridized to RNAs extracted from rat lung or rat penis and were then digested with a nuclease cocktail and electrophoresed on a polyacrylamide gel. The gel was exposed to film for autoradiography, and the signal intensity of the protected fragments on this assay demonstrated the relative abundance of the corresponding transcript. ampicillin […]

Similar primer sets were utilized to amplify cDNA that was reverse transcribed from human penis RNA and from RNA extracted from a human prostate cancer cell line, LNCaP, known to express VEGF-A in abundance. Figure 2B shows the ethidium bromide-stained agarose gel on which the amplification products from human penis cDNA, LNCaP cell cDNA, or […]

Each of these cDNA fragments was individually cloned into plasmids so that they could be sequenced and confirmed as VEGF splice variants. The sequencing confirmed our identification of the 188-, 164-, and 120-AA forms; it also confirmed that the 432-bp amplification product was the VEGF transcript variant encoding the 144-AA form of VEGF that had […]

Using a RT-PCR screening method, we attempted to identify the VEGF mRNA splice variants expressed in rat and human penile tissues. Oligonucleotide primers designed to amplify cDNA from the first to the eighth exon of rat VEGF were used in a PCR amplification reaction of cDNA made from RNA extracted from rat lung or from […]