Proteins Category

Collectively, these studies support the testable model depicted in Figure 14. As shown schematically, progesterone turns on transcriptional activation of the UG gene and also the alternative splicing event(s) that results in the preferential expression of RUSH-la. PRL signal transduction in uterine endometrium invokes a pathway in which PRL receptor binding activates a cascading series […]

Next it was important to determine whether PRL enhanced the progesterone-dependent increase in UG transcription through its action on the progesterone receptor alone, or whether the binding site for RUSH proteins is potentially important to the action of PRL. To test this, the full-length UG gene, pUG3.1-LUC, and the deletion mutant, pUG3.1 ARUSH-LUC, were used. […]

As shown in Figure 11 A, pUG3.1 contains all of the regions that have been suggested to be important in UG expression, especially the progesterone and estrogen receptor-binding sites, and the proximal promoter region known to bind RUSH. pUG2.3 contains the estrogen response element and the RUSH-binding site, but it lacks the progesterone receptor-binding site. […]

Because the sequence homology of the RUSH proteins with the SWI2/SNF2 multiprotein complex suggested a conserved role in transcription activation, it was not surprising to find that all male and female reproductive tract tissues, except for estrous uterine endometrium and lactat-ing mammary gland, and all non-reproductive tract tissues preferentially express the RUSH-la isoform (Table 1). […]

Rapid modifications in protein sequence/structure have the potential to quickly change transcription factor activity. Two such mechanisms are alternative splicing (posttran-scriptional) and phosphorylation (posttranslational). Alternative splicing, once thought to be the exception to the rule (one gene, one protein), is known to regulate approximately 5% of all genes. It is an economical way to produce […]

We next cloned, by recognition site screening, the cDNAs for two of the UG promoter-binding proteins (RUSH-la and RUSH-1. The RUSH acronym was derived from the first letter of a series of key characteristics as follows: each rabbit protein has the novel C3HC4 (RING) zinc-finger signature near its C-terminus; each protein is expressed in the […]

When the UG gene was analyzed for glucocorticoid and progesterone receptor-binding sites, none were found in the vicinity of the promoter. Rather, two strong and two weak progesterone receptor-binding sites were located between positions -2.7 kilobases (kb) and -2.3 kb (Fig. 8), and corresponded to DNase I hypersensitive regions in chromatin from endometrium of hormonally […]