Protein Category

Our data do support the hypothesis that icv IL-1p impairs StAR function, but that decreases in testicular synthesis of testosterone via inhibition of an enzyme (or enzymes) within the biosynthetic cascade that occurs within the mitochondria or smooth endoplasmic reticulum may also be involved. The second point is that, though present, the difference in the […]

Thus, though our data support a role for StAR, they suggest that icv injection of IL-1 p also alters the expression or activity of one or more of the downstream steroidogenic enzymes that synthesize testosterone from cholesterol. Since P450c17 is the most sensitive of the biosynthetic enzymes to IL-1p in vitro, we also examined the […]

On the basis of the foregoing observations, we had hypothesized that IL-1p administered into the cerebral ventricles might evoke changes in the capacity of Leydig cells to bind hCG/LH. Although we did observe a decrease in the binding of radiolabeled LH to cell membranes from rats treated in vivo with IL-1p (approximately 35%), there remained […]

Since one of the physiological changes evoked by hCG administration is increased blood flow through the testis, and since testicular blood flow is well correlated with the levels of testosterone in systemic circulation, it might have been hypothesized that the effect of icv-injected IL-1p on testicular function was mediated by alterations in testicular blood flow. However, […]

Our data show that in vivo administration of IL-1p into the cerebral ventricles blunts the capacity of the testis/Leydig cell to secrete testosterone ex vivo. This action is evident within 30 min of IL-1p administration, and the magnitude of the response is similar to that observed in preparations from rats exposed to IL-1p 90 min […]

Effect of In Vivo icv Treatment with Vehicle or Ii-1/3 on Expression of StAR Compared to vehicle treatment, in vivo exposure to IL-1p decreased levels of StAR protein detected in isolated Leydig cells within 30 min of injection (p = 0.0094, main effect, ANOVA). A longer interval between injection and killing of the animals did […]

Effect of In Vivo icv Treatment with Vehicle or IL-1/3 on Ex Vivo Secretion of Testosterone from Decapsulated Testes Although decapsulated testes from both vehicle and IL-1p-treated rats responded robustly to hCG stimulation ex vivo (p < 0.0001, ANOVA), prior exposure to the cytokine in vivo resulted in significant suppression of testosterone secretion at either […]