Phenotype Category

Several features of the overgrowth phenotype observed in Day 80 SCNT and IVF fetuses of the present study, e.g., disproportionate body and organ growth, normal number of placentomes but increased placentome size, have been reported previously but at later stages of gestation. Bovine IVF fetuses at Day 70 of gestation, in contrast, were not heavier […]

Development of Embryos Maturation rate of oocytes for nuclear transfer was 55%, and 203 of 215 matured and enucleated oocytes were successfully fused (94.4%) with nuclear donor cells. By Day 3, 139 KCCs (68.5%) had further developed; this resulted in 63 blastocysts (29.3%) by Day 7. In the IVF group, 120 oocytes subjected to maturation […]

Unless otherwise indicated, all chemicals were purchased from Sigma Chemical Co. (St. Louis, MO). Animals All germ cells and somatic cells used to generate fetuses were obtained from animals of the Brown Swiss breed. Oocytes for SCNT and IVF were obtained from slaughtered cattle identified by ID number. Nuclear donor granulosa cells were from the […]

Epigenetic modifications of the genome play a significant role in the elaboration of the genetic code and affect early growth and development through their influence on gene expression. Accumulating evidence indicates that incomplete or inappropriate epigenetic reprogramming of donor nuclei is a primary cause of low embryo production efficiency and high embryonic and fetal losses […]