Health Category

With the pig in the supine position, intravascular catheters were inserted into the jugular vein and femoral artery under surgical conditions. A 7.5F Swan-Ganz catheter (Baxter Healthcare Corporation; Santa Ana, CA) was placed into the pulmonary artery via the external jugular vein through a right cervical cutdown and a 3F polyethylene catheter (Plastimed; St. Leu […]

In the present study, we determined the profiles of CD38 expression and ADP-ribosyl cyclase activities during pregnancy. In myometrium obtained from term rats, there was increased CD38 mRNA and protein expression compared with levels at Days 14-17 of pregnancy. This increased CD38 protein expression was associated with significantly increased ADP-ribosyl cyclase, but not cADPR hydrolase, […]

CD38 Expression in Rat Uterine Smooth Muscle During Gestation CD38 mRNA expression was determined in myometrium obtained from preterm and term rats. In uterine smooth muscle obtained from term rats, there was increased CD38 mRNA expression compared with smooth muscle from preterm rats (Fig. 1A). Radiometric analysis of the intensity of the bands showed that […]

Materials Tris Base, Tris-HCl, Glucose, HEPES, nicotinamide guanine dinucleotide (NGD), cyclic guanosine diphosphoribose (cGDPR), 1,1,2,-trichlo-rotrifluoroethane, tri-n-octylamine, and other chemicals were purchased from Sigma Chemical Company (St. Louis, MO). Hanks balanced salt solution (HBSS), hexamers, oligo(dT), Taq DNA polymerase, and 100base pair (bp) DNA ladder were purchased from GibcoBRL (Grand Island, NY). Cellulose polyethyleneimine (PEI) thin-layer […]

Cyclic ADP-ribose (cADPR), a metabolite of p-NAD, mobilizes calcium from intracellular stores such as sarcoplasmic reticulum (SR) through ryanodine receptor (RyR) channels in a variety of cell types, including smooth muscle cells. Synthesis of cADPR from p-NAD is catalyzed by ADP-ribosyl cyclase, and cADPR is hydrolyzed to ADPR by cADPR hydrolase. In mammalian cells, both […]

Oocyte Isolation Ovaries were placed in 1 ml М2 medium in a 30-mm Petri dish (Falcon, Becton & Dickinson, Oxford, UK) under a stereomicroscope (X30), and their antral follicles were punctured with a thin and sharp pulled sterile Pasteur pipette until oocytes were released into the medium. Those oocytes that still showed the presence of […]

Cell culture. Osteosarcoma ROS 17/2.8 cells were cultured ac­cording to previously published protocols. Briefly, ROS 17/2.8 cells (kindly provided by M.C. Farach-Carson, University of Delaware) were grown in DMEM/Ham’s F-12 (50:50, v/v) supplemented with 10% fetal bovine serum and antibiotics. Cell cultures were kept in a humidified atmosphere containing 5% CO2 at 37°C. For patch-clamp […]