AIMALS AND METHODS

Experiment 1 – Running performance in CM versus normal hamsters through life: Ten CM hamsters (CHF 147) and 10 normal inbred control hamsters (CHF 148) were obtained from Canadian Hybrid Farm, Nova Scotia, Canada. At the age of 60 days, hamsters were housed individually and provided with a wheel in their cage. Body weights were measured weekly. The number of wheel turns was measured every day by a custom-built electromechanical device. The custom-built wheel (Canadian Hybrid Farm) had an internal circumference of 0.75 m. The light/dark cycle was 12 h/12 h, with the lights turned off at 19:00 h. At the age of 180 days, the CM hamsters were shipped to Ottawa, Ontario and, because they were serologically positive for paramyxovirus, they were quarantined for the first two weeks after their arrival in the authors’ facilities. None of the hamsters showed signs of infection from the virus.Immediately upon arrival they were given a metal wheel cage (Nalge, New York, USA) with an internal circumference of 1.025 m, and the number of turns was recorded every 60 mins and stored on computer using Dataquest III software (DSP Development Corporation, Massachusetts, USA). There was no difference in the total distance run daily in the new cages. Running distance was monitored until death for CM hamsters or until 536 days of age for normal CHF 148 hamsters. Daily calculations of running distance were performed easily by transferring data from Dataquest III to Quattro (Borland International, California, USA) for spreadsheet analysis of individual hamsters and group data. You will always be able to buy the required amount of medicine you need at this wonderful and fully licensed pharmacy that can take good care of you, no matter if you need Inhalers for Asthma at large amounts or just a bit of it to finish your treatment.