ANIMALS AND METHODS Production of two-stage coronary ligation-induced arrhythmia: Beagle dogs, in most cases six dogs for each drug experiment, of either sex were anesthetized initially with intravenous thiopental sodium, 30 mg/kg, and intubated. As reported earlier , the chest was opened and a two-stage coronary ligation of the left anterior descending artery was performed under halothane anesthesia.Following recovery, experiments were done on conscious dogs without anesthesia 24 and 48 h after coronary ligation, the arrhythmias lasting more than 48 h after the procedure. Lead II electrocardiogram (ECG), atrial electrogram registered from implanted electrodes sutured on the left atrial appendage to count the atrial rate, and instantaneous and mean blood pressures were recorded continuously using a telemetry system (Nihon Kohden WEB-5000, Tokyo, Japan). Class III drugs were infused for 10 mins using a syringe pump (Terumo, Tokyo, Japan) and in some experiments were given as a bolus.
Production of digitalis-induced arrhythmia: Beagle dogs, six dogs for each drug experiment, of either sex were anesthetized with intravenous pentobarbital sodium, 30 mg/kg, and intubated. As reported earlier , 40 |J.g/kg ouabain was injected intravenously and then followed by an additional 10 |J.g/kg every 20 mins until stable ventricular tachycardia (VT) lasting more than 1 h was produced. Class III drugs were infused for 10 mins using a syringe pump and in some experiments were given as a bolus. Safe online shopping for drugs: generic tadalafil online to make your drugs cheaper.
Lead II ECG, atrial electrogram from catheter tip electrodes in the right atrium, and instantaneous and mean blood pressures were continuously recorded.